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KMID : 0370219830270040249
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Yakhak Hoeji
1983 Volume.27 No. 4 p.249 ~ p.256
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Effect of Phospholipase D on the L-alpha-Dimyristoyl-phosphatidyl Choline Liposome Containing Cholesterol, L-alpha-Phosphatidylinositol and L-alpha-Phosphatidylserine
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이은옥/Lee EO
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Abstract
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When the reaction rate constant k of phospholipase D on liposome was measured by the ANS fluorometry, k of phospholipase D on DMPC liposome which was made of L-alpha-PI, cholesterol and L-alpha-PS decreased than that of phospholipase D on DMPC liposome with cholesterol or with PI and cholesterol. Optimal Ca2+ concentration, the most important factor on effect of phospholipase D, also decreased to 1mM, as compared with 1OmM and 6OmM respectively when cholesterol and PI were added, and cholesterol only was added. The change of cholesterol Mol% had a great influence on k value of phospholipase D. But in case of addition of L-alpha-PS to cholesterol, the influence was relatively diminished.
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KEYWORD
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